Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path

Table 1 Identification of the number of hybridizing colonies (BAC clones) and inferred significantly conserved paralogues (G) identified by probes (P) to 201 small gene families in soybean. MTP BAC DNA was hybridized to 166 EST probes from cv. Williams (Gm library) and 143 EST probes from cv. Forrest (Fi library). Hybridization stringency was about 25 C below Tm so paralogue detected were expected to share more than 75% nucleotide identity.

	Probe	Number of paralogues per gene family
Library	total	1	2	3	4	5	6	7	8	9	10	13	15
Fi-P	101	34	20	14	9	5	5	5	2	2	3	1	1
Fi-G	334	34	40	42	36	25	30	35	16	18	30	13	15
Gm-P	100	36	29	7	12	4	5	2	2	0	1	2	0
Gm-G	279	36	58	21	48	20	30	14	16	0	10	26	0
Total-P	201	70	49	21	21	9	10	7	4	2	4	3	1
Total-G	613	70	98	63	84	45	60	49	32	18	40	39	15

ISSN: 1746-4811