Figure 4

Rapid selection of hygromycin B-resistant Arabidopsis thaliana Col-0 seedlings. a. Seedlings from Arabidopsis thaliana Col-0 T1 seed obtained from plants subjected to floral dip transformation using Agrobacterium tumefaciens strain GV3101 harbouring the binary plasmid pBIG-HYG. Seeds were plated on 1% agar containing MS medium (including vitamins) and hygromycin B at a concentration of 15 μg.ml^-1. Following a stratification period of 2 d at 4°C in the dark, seedlings were incubated for 6 h at 22°C in white light, followed by incubation at 22°C in the dark for 2 d and continuous white light for a further 24 h. b. Hygromycin B-resistant Arabidopsis seedling after rapid selection procedure (left); kanamycin-susceptible seedling after rapid selection procedure (right). Size bars 1 cm.