Skip to main content
Figure 2 | Plant Methods

Figure 2

From: A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation

Figure 2

Rapid selection of kanamycin-resistant Arabidopsis thaliana Col-0 seedlings. a. Seedlings from Arabidopsis thaliana Col-0 T1 seed obtained from plants subjected to floral dip transformation using Agrobacterium tumefaciens strain GV3101 harbouring the binary plasmid pBINPLUS. Seeds were plated on 1% agar containing MS medium (including vitamins) and kanamycin at a concentration of 50 μg.ml-1. Following a stratification period of 2 d at 4°C in the dark, seedlings were incubated for 6 h at 22°C in white light, followed by incubation at 22°C in the dark for 2 d and continuous white light for a further 24 h. b. Kanamycin-resistant Arabidopsis seedling after rapid selection procedure (left); kanamycin-susceptible seedling after rapid selection procedure (right). Size bars 1 cm.

Back to article page

Plant Methods

ISSN: 1746-4811