Table 1 Sensitivity of FISH to detect single or low copy genes or transgenes.
Plant Species | Target DNA | Phase of Cell Cycle | Probe size | Reference |
|---|---|---|---|---|
Petroselium crispum | Endogenous | Metaphase | 6,6 Kb | [104] |
Oryza sativa | T-DNA | Metaphase | 5,6 Kb | [3,105] |
Oryza sativa | T-DNA | Metaphase | 5,5 Kb | [106] |
Zea mays | T-DNA | Meiosis (pachytene) | 3,1 Kb | [107] |
Zea mays | Endogenous | Meiosis (pachytene) | 3,1 Kb | [108] |
Petunia hybrida | T-DNA | Metaphase | 2.7 Kb | [46] |
Triticum aestivum | Bombardment | Interphase nuclei and metaphase chromosomes | 1.8 Kb | [2,14] |
Asparagus officinalis | Endogenous | Interphase nuclei and metaphase chromosomes | 1,4 Kb/1,7 Kb | [109] |
Oryza sativa | Endogenous | Metaphase | 1,29 Kb | [110] |
Petunia hybrida | T-DNA | Metaphase | 4 Kb | [36] |
Allium cepa | T-DNA | Metaphase | 710 bp | [49] |
Beta vulgaris | Endogenous | Metaphase | 684 bp | [48] |