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Table 1 Sensitivity of FISH to detect single or low copy genes or transgenes.

From: In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research

Plant Species Target DNA Phase of Cell Cycle Probe size Reference
Petroselium crispum Endogenous Metaphase 6,6 Kb [104]
Oryza sativa T-DNA Metaphase 5,6 Kb [3,105]
Oryza sativa T-DNA Metaphase 5,5 Kb [106]
Zea mays T-DNA Meiosis (pachytene) 3,1 Kb [107]
Zea mays Endogenous Meiosis (pachytene) 3,1 Kb [108]
Petunia hybrida T-DNA Metaphase 2.7 Kb [46]
Triticum aestivum Bombardment Interphase nuclei and metaphase chromosomes 1.8 Kb [2,14]
Asparagus officinalis Endogenous Interphase nuclei and metaphase chromosomes 1,4 Kb/1,7 Kb [109]
Oryza sativa Endogenous Metaphase 1,29 Kb [110]
Petunia hybrida T-DNA Metaphase 4 Kb [36]
Allium cepa T-DNA Metaphase 710 bp [49]
Beta vulgaris Endogenous Metaphase 684 bp [48]