Figure 3

Transgene sites visualized in tobacco isolated nuclei. In panels A and B a single nucleus is shown, stained with DAPI (3A), and labeled by FISH (3B). This nucleus originates from a double haploid tobacco transgenic plant line that contains 7 copies of the GUS gene [63]; two signals, each corresponding to a homologous chromosome, are clearly visible (arrows). In panels C and D two isolated nuclei from independent NT1 tobacco suspension cell lines expressing luciferase are shown [65]. Panel C demonstrates the detection of a single copy luciferase gene using a luc fragment probe. Panel D shows a nucleus with multiple insertions in a total of 48 transgene copies. Bar, 10 μm.