Table 1 pOy vectors
Vector | Description | Residue alterations | Protein detected | Phenotype |
|---|---|---|---|---|
pOY-1 | ZmChlI wild-type | - | yes | none |
pOY-2 | ZmChlI-Oy1-N1989 | ΔAT63 L176F | yes | chlorosis |
pOY-3 | ZmChlI-Oy1-N1989 derivative | ΔAT63 | yes | none |
pOY-4 | ZmChlI-Oy1-N1989 derivative | L176F | yes | chlorosis |
pOY-5 | ZmChlI-Oy1-N700 | E243K | yes | chlorosis |
pOY-10 | SsChlI wild-type, N-terminal CTP fusion | ZmCHLI(1–54)-SsCHLI | yes | chlorosis |
pOY-11 | SsChlI-L113F, N-terminal CTP fusion | ZmCHLI(1–54)-SsCHLI-L113F | yes | chlorosis |
pOY-12 | SsChlI-L113F, no CTP fusion | L113F | no | none |