Novel Zm ChlI alleles obtained from PCR-based mutagenesis. A) Protein alignments showing lesions resulting from PCR-based mutagenesis. Recovered alleles compared to wild-type CHLI sequence from Zea mays (AAZ14052), Hordeum vulgare (DQ529207), Arabidopsis thaliana (NP_193583), Nicotiana tabacum (AAB97153), Synechocystis sp. (BAA17166), and Rhodobacter sphaeroides (ZP_00918218). Amino acid substitutions are denoted with the position of the change relative to the wild-type ZmCHLI sequence. B) Leaves of N. benthamiana 12 days post-infiltration.