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Table 4 Measured ratios of 15N/14N forms of metabolite fragment peaks in 1:1 mixtures of labeled and unlabeled extracts compared to their expected values. Expected ratios were calculated based on the fragment spectra ratios in the unmixed extracts (14N-extract, 15N-extract). The difference of the measured values to the expected values is expressed as percentage of the expected value.

From: Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites

 

measured ratios

expected ratios

difference to expected (%)

ala

0.31

0.11

191.3

asp

7.52

7.29

3.2

cys

1.29

1.51

-14.4

glu

1.05

0.86

22.1

gly

1.04

0.93

11.9

ile

2.43

2.36

3. 0

lys

1.27

1.24

2.8

met

3.91

44.54

-91.2

phe

2.31

2.28

1.5

pro

2.54

2.49

1.9

thr

0.53

0.44

19.8

trp

2.51

2.55

-1.7

tyr

1.82

1.97

-7.8

val

1.54

1.43

7.5

put

7.16

9.48

-24.5

spe

1.03

0.97

7.0