Skip to main content

Table 4 Measured ratios of 15N/14N forms of metabolite fragment peaks in 1:1 mixtures of labeled and unlabeled extracts compared to their expected values. Expected ratios were calculated based on the fragment spectra ratios in the unmixed extracts (14N-extract, 15N-extract). The difference of the measured values to the expected values is expressed as percentage of the expected value.

From: Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites

  measured ratios expected ratios difference to expected (%)
ala 0.31 0.11 191.3
asp 7.52 7.29 3.2
cys 1.29 1.51 -14.4
glu 1.05 0.86 22.1
gly 1.04 0.93 11.9
ile 2.43 2.36 3. 0
lys 1.27 1.24 2.8
met 3.91 44.54 -91.2
phe 2.31 2.28 1.5
pro 2.54 2.49 1.9
thr 0.53 0.44 19.8
trp 2.51 2.55 -1.7
tyr 1.82 1.97 -7.8
val 1.54 1.43 7.5
put 7.16 9.48 -24.5
spe 1.03 0.97 7.0