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Table 5 Overview of the costs of SILAC labeling and full 15N-labeling for plant cell culturesa. Depending on the compounds and concentrations used in the medium, K15NO3 labeling is 7 to 12 times cheaper per liter medium.

From: Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites

Compound

Amount in package (g)

Cost per package (€)

Cost per g (€)

Final concentration in medium (mM)

Price per L (€)

Cost Factor

13C6-R

0.100

750 b

7500

0.8 c

972

13

13C6-K

0.100

800 b

8000

0.8 c

1037

13

D3-L

0.100

550 b

5500

0.8 c

590

8

K15NO3

1

75 b

75

10 d

77

1

  1. a for comparison, the iTRAQ Multiplex Kit costs approximately 1000 € (Applied Biosystems, Germany), allowing for 10 pairwise labelling reactions.
  2. b approximate prices according to SigmaAldrich, Germany
  3. c most efficient concentration used in Gruhler et al. 2005, MCP [35]
  4. d as used in this study
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Plant Methods

ISSN: 1746-4811