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Table 5 Overview of the costs of SILAC labeling and full 15N-labeling for plant cell culturesa. Depending on the compounds and concentrations used in the medium, K15NO3 labeling is 7 to 12 times cheaper per liter medium.

From: Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites

Compound Amount in package (g) Cost per package (€) Cost per g (€) Final concentration in medium (mM) Price per L (€) Cost Factor
13C6-R 0.100 750 b 7500 0.8 c 972 13
13C6-K 0.100 800 b 8000 0.8 c 1037 13
D3-L 0.100 550 b 5500 0.8 c 590 8
K15NO3 1 75 b 75 10 d 77 1
  1. a for comparison, the iTRAQ Multiplex Kit costs approximately 1000 € (Applied Biosystems, Germany), allowing for 10 pairwise labelling reactions.
  2. b approximate prices according to SigmaAldrich, Germany
  3. c most efficient concentration used in Gruhler et al. 2005, MCP [35]
  4. d as used in this study