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Table 3 A subset of tryptic peptides from glyceraldehyde-3-phosphate dehydrogenase C subunit (At3g04120) that were identified by mass spectrometry and subsequent database search (14N-search, see methods for details). For each peptide the expected mass difference was calculated based on the measured mass to charge ratio (MCR). Asterisks mark those peptides that have also been identified in the database search with 15N as fixed modification (15N-search, see Methods for details)

From: Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites

Sequence

measured mass 14N

MCR 14N [Th]

Charge

calculated mass difference

calculated mass 15N

MCR 15N [Th]

AASFNIIPSSTGAAK

1434.42

718.22

2

16.95

1451.37

726.69

DAPMFVVGVNEHEYK

1733.42

867.72

2

18.94

1752.36

877.18

FGIVEGLMTTVHSITATQK *

2033.69

1017.85

2

18.94

2052.63

1027.32

GILGYTEDDVVSTDFVGDNR

2170.55

1086.28

2

23.93

2194.48

1098.24

LVSWYDNEWGYSSR

1760.47

881.24

2

19.94

1780.41

891.20

SDLDIVSNASCTTNCLAPLAK

2248.71

1125.36

2

23.93

2272.64

1137.32

VPTVDVSVVDLTVR *

1497.55

749.78

2

16.95

1514.50

758.25

YDSVHGQWK

1118.39

560.20

2

13.96

1132.35

567.17