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Figure 2 | Plant Methods

Figure 2

From: Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites

Figure 2

Ion intensities of characteristic fragment ions for 14N and 15N isoforms of metabolites in unlabeled cells (14N), 15N-labeled cells (15N) and in extracts of 1:1, 1:4, and 4:1 mixtures of unlabeled to labeled cells is shown for the amino acid glycine (A), the amino acid threonine (B), the amino acid glutamic acid (C), and the polyamine spermidine (D). Non-N-containing compounds inositol (D) and fumarate (E) were used as a control for equal extract material. Each mixture was measured in five independent samples, mean of five replica ± standard deviation are shown, and ratios between 15N-form and 14N-form for each cell mixture are shown above the bars. Average labeling efficiency of all N-containing metabolites was 91.32% as estimated from the 15N to 14N ratios in the 15N-labeled extract.

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