Table 1 Plasmid constructs and siRNAs summary. A list of plasmid and siRNA size, description and origin.
From: A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts
Name | Nucleic acid type | Size (bp) | Description | Origin |
|---|---|---|---|---|
pLUC | Plasmid | ~5500 | Cauliflower mosaic virus (CaMV)35S promoter-luciferase GL2 | [23] |
p35S-GFP | Plasmid | ~4500 | 35S (CaMV)-EGFP | [24] |
pUbi-GFP | Plasmid | ~12000 | smGFP in Ubiquitin (Ubi)-pCambia 1300 backbone | This study |
pGUS | Plasmid | ~4500 | Ubi-GUS | [22] |
siLUC | siRNA | 21 | siRNA targeting luciferase GL2 | Qiagen, USA |