Figure 6
From: A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts
Transformation and silencing in protoplasts prepared from green tissue. Protoplasts were prepared as described (Methods). Protoplasts were suspended in Mmg solution at a concentration of 5 × 106 cells/ml. For transformation, 5 μg pLUC and 5 μg pGUS (as an internal control) were combined with 100 μl (5 × 106 cells/ml) of protoplasts and with or without 3 μg siLUC. Luciferase activity was measured as described 16 h after transformation.