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Table 1 Examples of plant protein-protein interactions studied via FRET or BiFC.

From: The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods

Protein-protein interaction Applied technique Cell type Gene transfer method reference
Phytochrome B-Cryptochrome 2 FRET (channel and DFRAP) Tobacco protoplasts Protoplast transfection [24]
SERK-1 (homodimerization) FRET (FSPIM) Cowpea mesophyll protoplasts Protoplast transfection [25]
Floral binding protein 2 (homodimerization) Floral binding protein 11 FRET (FSPIM and FLIM) Petunia leaf protoplasts Protoplast transfection [26]
TGA5 (homodimerization) FRET (channel) Tobacco leaf cells Agroinfiltration [27]
SERK1-KAPP FRET (FSPIM) Cowpea mesophyll protoplasts Protoplast transfection [28]
Opaque2-CGN5/ADA2 FRET (DFRAP and FLIM) Cowpea mesophyll protoplasts Protoplast transfection [29]
Lipidated YFP and CFP variants FRET (FSPIM and FLIM) Cowpea protoplasts Protolplast transfection [30]
AtMinD1 (homodimerization) FRET (channel and DFRAP) Tobacco leaf epidermal cells Particle bombardment [31]
MLO-calmodulin FRET (DFRAP and FLIM) Barley leaf epidermal cells Particle bombardment [32]
MLO (homodimerization) FRET (DFRAP) Barley leaf epidermal cells Particle bombardment [33]
Vacuolar ATPase subunits FRET (channel) Arabidopsis leaf mesophyll protoplasts Protoplast transfection [34]
MADS box proteins FRET (FLIM) Cowpea and Petunia leaf protoplasts Protoplast transfection [35]
SAG101-EDS1 FRET (DFRAP) Arabidopsis leaf epidermal cells Particle bombardment [36]
AtMinE1-AtMinD1, AtFtsZ1-1-AtFtsZ2-1, AtFtsZ2-1-ARC6 FRET (method unknown) Tobacco leaf epidermal cells Particle bombardment [37]
bZIP63 (homodimerization) BiFC Tobacco leaf epidermal cells Agro-infiltration [68]
LSD1 (homodimerization) BiFC Arabidopsis leaf epidermal cells Agro-infiltration [68]
14-3-3 (homodimerization) BiFC Arabidopsis cell culture protoplasts and tobacco leaf epidermal cells Protoplast transfection and Agro-infiltration [68]
PFTα-PFTβ BiFC Arabidopsis leaf epidermal cells Agro-infiltration [69]
FIE-MEA BiFC Tobacco and Arabidopsis leaf epidermal cells Agro-infiltration [69]
VIP1-VirE2, VIP1-VirF BiFC Tobacco and onion leaf epidermal cells Particle bombardment [70]
SAD-GAMYB BiFC Onion leaf epidermal cells Particle bombardment [71]
OFP1 (homodimerization), BLH1 (homodimerization), AtOFP1-AtOFP1 BiFC Tobacco leaf cells Agroinfiltration [72]
VirE2-VirE3 BiFC Tobacco and onion leaf epidermal cells Particle bombardment [73]
VIP1-VirE2 BiFC Tobacco leaf epidermal cells Particle bombardment [74]
VIP1-H2A BiFC Tobacco leaf epidermal cells Particle bombardment [75]
EID1-ASK1 BiFC Mustard seedlings and parsley protoplasts Particle bombardment and protoplast transfection [76]
FD-FT BiFC Tobacco leaf epidermal cells Agro-infiltration [77]
AtMinE1-AtMinD1, AtFtsZ1-1-AtFtsZ2-1, AtFtsZ2-1-ARC6 BiFC Tobacco leaf epidermal cells Particle bombardment [37]
OsOBF1 (homodimerization), OsOBF1-LIP19 BiFC Onion bulb epidermal cells Particle bombardment [78]
ATH1-STM, BLH3-STM, BLH9-STM BiFC Leek epidermal cells Particle bombardment [79]
p6 and TGBp2 topology BiFC Tobacco leaf epidermal cells Particle bombardment and Agro-infiltration [80]