Table 1 Examples of plant protein-protein interactions studied via FRET or BiFC.
Protein-protein interaction | Applied technique | Cell type | Gene transfer method | reference |
|---|---|---|---|---|
Phytochrome B-Cryptochrome 2 | FRET (channel and DFRAP) | Tobacco protoplasts | Protoplast transfection | [24] |
SERK-1 (homodimerization) | FRET (FSPIM) | Cowpea mesophyll protoplasts | Protoplast transfection | [25] |
Floral binding protein 2 (homodimerization) Floral binding protein 11 | FRET (FSPIM and FLIM) | Petunia leaf protoplasts | Protoplast transfection | [26] |
TGA5 (homodimerization) | FRET (channel) | Tobacco leaf cells | Agroinfiltration | [27] |
SERK1-KAPP | FRET (FSPIM) | Cowpea mesophyll protoplasts | Protoplast transfection | [28] |
Opaque2-CGN5/ADA2 | FRET (DFRAP and FLIM) | Cowpea mesophyll protoplasts | Protoplast transfection | [29] |
Lipidated YFP and CFP variants | FRET (FSPIM and FLIM) | Cowpea protoplasts | Protolplast transfection | [30] |
AtMinD1 (homodimerization) | FRET (channel and DFRAP) | Tobacco leaf epidermal cells | Particle bombardment | [31] |
MLO-calmodulin | FRET (DFRAP and FLIM) | Barley leaf epidermal cells | Particle bombardment | [32] |
MLO (homodimerization) | FRET (DFRAP) | Barley leaf epidermal cells | Particle bombardment | [33] |
Vacuolar ATPase subunits | FRET (channel) | Arabidopsis leaf mesophyll protoplasts | Protoplast transfection | [34] |
MADS box proteins | FRET (FLIM) | Cowpea and Petunia leaf protoplasts | Protoplast transfection | [35] |
SAG101-EDS1 | FRET (DFRAP) | Arabidopsis leaf epidermal cells | Particle bombardment | [36] |
AtMinE1-AtMinD1, AtFtsZ1-1-AtFtsZ2-1, AtFtsZ2-1-ARC6 | FRET (method unknown) | Tobacco leaf epidermal cells | Particle bombardment | [37] |
bZIP63 (homodimerization) | BiFC | Tobacco leaf epidermal cells | Agro-infiltration | [68] |
LSD1 (homodimerization) | BiFC | Arabidopsis leaf epidermal cells | Agro-infiltration | [68] |
14-3-3 (homodimerization) | BiFC | Arabidopsis cell culture protoplasts and tobacco leaf epidermal cells | Protoplast transfection and Agro-infiltration | [68] |
PFTα-PFTβ | BiFC | Arabidopsis leaf epidermal cells | Agro-infiltration | [69] |
FIE-MEA | BiFC | Tobacco and Arabidopsis leaf epidermal cells | Agro-infiltration | [69] |
VIP1-VirE2, VIP1-VirF | BiFC | Tobacco and onion leaf epidermal cells | Particle bombardment | [70] |
SAD-GAMYB | BiFC | Onion leaf epidermal cells | Particle bombardment | [71] |
OFP1 (homodimerization), BLH1 (homodimerization), AtOFP1-AtOFP1 | BiFC | Tobacco leaf cells | Agroinfiltration | [72] |
VirE2-VirE3 | BiFC | Tobacco and onion leaf epidermal cells | Particle bombardment | [73] |
VIP1-VirE2 | BiFC | Tobacco leaf epidermal cells | Particle bombardment | [74] |
VIP1-H2A | BiFC | Tobacco leaf epidermal cells | Particle bombardment | [75] |
EID1-ASK1 | BiFC | Mustard seedlings and parsley protoplasts | Particle bombardment and protoplast transfection | [76] |
FD-FT | BiFC | Tobacco leaf epidermal cells | Agro-infiltration | [77] |
AtMinE1-AtMinD1, AtFtsZ1-1-AtFtsZ2-1, AtFtsZ2-1-ARC6 | BiFC | Tobacco leaf epidermal cells | Particle bombardment | [37] |
OsOBF1 (homodimerization), OsOBF1-LIP19 | BiFC | Onion bulb epidermal cells | Particle bombardment | [78] |
ATH1-STM, BLH3-STM, BLH9-STM | BiFC | Leek epidermal cells | Particle bombardment | [79] |
p6 and TGBp2 topology | BiFC | Tobacco leaf epidermal cells | Particle bombardment and Agro-infiltration | [80] |