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Figure 1 | Plant Methods

Figure 1

From: The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods

Figure 1

Excitation and emission spectra of a commonly used FRET pair. The scheme depicts simplified absorbance and emission spectra of CFP (cyan fluorescent protein; donor; D) and YFP (yellow fluorescent protein; acceptor, A). Overlap between CFP emission and YFP absorption (shaded region) is a prerequisite for FRET. Dabs – Donor absorbance; Dem – Donor emission; Aabs – Acceptor absorbance; Aem – Acceptor emission.

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Plant Methods

ISSN: 1746-4811