Figure 3

Assessment of basal heat tolerance in Col seeds. (A-D) Seeds were subjected to 50°C heat shock for different time periods (0–300 min). (A) Radicle emergence and (B) green cotyledon emergence during the 12 day post-heat shock period. (C) Germination levels (radicle emergence parameter) after 12 days, plotted as a function of treatment period. (D) Morphology of 12-day-old seedlings. (E-H) Seeds were subjected to a 60 min heat shock in a range of different temperatures (40-57°C). (E) Radicle emergence and (F) green cotyledon emergence during the 12 day post-heat shock period. (G) Germination levels (radicle emergence parameter) after 12 days, plotted as a function of heat-shock temperature. (H) Morphology of 12-day-old seedlings. Percentages were determined in relation to the total number of sown seeds. Error bars represent SEM (n = 5 plates with ~20 seedlings each).