Figure 3

Dose-dependent ROS production in response to live Pto DC3000 and Pto hrcC^-in wildtype Col-0. A: Time-course of ROS production in response to Pto DC3000 cells with an OD₆₀₀ of 0.001, 0.01 and 0.1. B: Time-course of ROS production in response to Pto hrcC^- cell with an OD₆₀₀ of 0.001, 0.01 and 0.1. C: Comparison of peak ROS production for each concentration of Pto DC3000 (black bar) or Pto hrcC^- (white bar) shown at the point of maximum ROS production 35 minutes post-elicitation). For ROS production, Col-0 leaf disk halves were elicited with DC3000 (filled shape) or hrcC^- (open shapes) with the indicated bacterial cell density at 0 min (n = 16/treatment). To allow direct comparisons, all ROS experiments (A-C) were performed in the same 96-well plate at the same time. Values are mean ± SE, means with different letters denote a significance difference while similar letters denote no significance (Two tailed student’s t-test, P < 0.005). Experiments were repeated more than 3 times with similar results. RLU, Relative Light Units; OD, Optical density.