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Figure 1 | Plant Methods

Figure 1

From: Rapid bioassay to measure early reactive oxygen species production in Arabidopsis leave tissue in response to living Pseudomonas syringae

Figure 1

Outline for plate-setup and preparation of bacterial elicitation solution to measure luminol-based ROS production in leaf disc halves of Arabidopsis wildtype (Col-0) and mutant lines in response to live bacterial Pto cells. A: Preparation of a 96-well assay plate containing leaf disc halves of wildtype (WT, white) and mutant Arabidopsis‚ÄČlines (mut 1, gray and mut 2, black). B: Preparation of Elicitation Solution containing live Pto cells. C: Elicitation of wild-type and mutant tissue with Elicitation Solution to measure ROS response. WT, wild-type; mut, mutant; OD, optical density.

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