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Table 2 Gene specific primers used for the amplification of targeted genes OsACS1 and OsSGR design for target genes amplification

From: EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations

Target gene

Primer name

Nucleotide sequences 5′-3′

Amplicon size (bp)

Amplicon name

OsACS1

ACS1 1–3 F

TAAGCAGCTCGTCCAACCTT

1014

ACS1 1-3

ACS1 1–3 R

CAGTGCACGGGTACGATCT

ACS1 4 F

CTCATCCCCACCCCATACTA

1480

ACS1 4

ACS1 4 R

CCCAAATGTGGGAGTGGTAG

OsSGR

SGR 1–2 F

TAAGAGATCCGAGGGAGCAG

926

SGR

SGR 1–2 F

ACAGATGGATGGATGCCAAT

  1. Primers were designed using Primer3 application and used for the OsACS1 (Os03g0727600) and OsSGR (Os09g0532000) molecular screening of mutations. F: forward. R: reverse. Position of amplicons are shown in Figure 2.