Table 1 Mutations discovered in the Oryza sativa cv. Hispagran population obtained from 0.2% EMS-mutagenised callus
Mutant gene | Mutant code | Target fragment | Nucleotide change | Gene bp position | Effect | Predicted protein structure modification |
|---|---|---|---|---|---|---|
acs1 | 152 s3 | ACS1 1-3 | T- > G | 58 | C20G | Exon 1 α-helix domain modification (weak effect) |
acs1 | 418 s2 | ACS1 1-3 | C– > T | 84 | S28S | Exon 1 silent mutation |
acs1 | 152 s3 | ACS1 1-3 | G- > A | 174 | GT- > AT | Intron 1 5′UTR splicing donor site GT modification that may affect splicing |
acs1 | 558 s2 | ACS1 1-3 | G- > A | 535 | Q114Q | Exon 3 silent mutation |
acs1 | 43 s3 | ACS1 1-3 | T- > A | 707 |  | Intron 3 mutation |
acs1 | 83 s2 | ACS1 4 | C– > T | 1177 | F231F | Exon 4 silent mutation |
acs1 | 398 s4 | ACS1 4 | G- > C | 1220 | A246P | Exon 4 α-helix domain lost |
acs1 | 228 s1 | ACS1 4 | G- > A | 1266 | S314N | Exon 4 α-helix domain modification (+1) |
acs1 | 576 s1 | ACS1 4 | C– > T | 1351 | L354P | Exon 4 α-helix domain modification affecting functional domain |
acs1 | 364 s2 | ACS1 4 | G- > A | 1669 | L395L | Exon 4 silent mutation |
acs1 | 408 s3 | ACS1 4 | C– > T | +73 |  | Downstream mutation |
sgr | 24 s1 | SGR9 1-2 | G- > A | 67 | A23T | Exon 1 α-helix domain modification (weak effect) |
sgr | 855 s2 | SGR9 1-2 | C- > T | 78 | L26L | Exon 1 silent mutation |
sgr | 854 s1 | SGR9 1-2 | G- > A | 238 |  | Intron 1 mutation |
sgr | 389 s2 | SGR9 1-2 | G- > A | 484 | V127M | Exon 2 α-helix domain modification (+2) |