Skip to main content
Figure 2 | Plant Methods

Figure 2

From: EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations

Figure 2

Plantlets regeneration of Oryza sativa var. Hispagram from EMS mutagenised calli. (a) Oryza sativa cv. Hispagran dehusked seeds forming callus masses after 18 days culture in darkness using N6 medium supplemented with 0.5 mg L-1 casaminoacids, 1 g L-1 L-proline, 2 mg L-1 2,4 dichlorophenoxyacetic acid and 0.5 g L-1 2-(N-morpholino) ethane sulphonic acid. (b)  development of a scutellum-derived callus mass (black arrow) and a callus mass growing from radicle (black circle), (c) and (d) plantlet regeneration from mature seed-derived callus (0.2% EMS mutagenised) in MS medium supplemented with 1 g L-1 casein hydrolisate, 3 mg L-1 kinetin, 0.5 mg L-1  6-benzylaminopurin, 0.5 mg L-1 1-naphtalenacetic acid and 0.5 g L-1 2-(N-morpholino) ethane sulphonic acid under 18/6 h light/dark cycles (details in Methods). Scale bar 1 cm.

Back to article page

Plant Methods

ISSN: 1746-4811