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Figure 1 | Plant Methods

Figure 1

From: EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations

Figure 1

Diagram comparing callus mutagenesis and seed mutagenesis protocols in rice TILLING. (a) In the proposed rice TILLING protocol through callus mutagenesis, calli are induced, mutagenised, and the regenerated plants provide DNA for molecular screening of mutations. (b) In the basic TILLING method, seeds are mutagenised, the resulting M1 plants are self-fertilized and the M2 generation of individuals is used to prepare DNA samples for mutational screening while their seeds are inventoried. The steps represented cover from callus induction (a) or seed imbibition (b) to DNA extractions for molecular screening of mutations. Duration of each step is indicated.

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Plant Methods

ISSN: 1746-4811