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Table 3 Matrix effect of plant tissue analyzed by MSPE-ISD

From: Improved methodology for assaying brassinosteroids in plant tissues using magnetic hydrophilic material for both extraction and derivatization

Analytes Matrix effect/%
28-norBL 85.9
BL 67.4
28-homoBL 76.0
28-norCS 93.1
CS 77.8
  1. Matrix effect = BR/IS peak area ratio of the real sample over the BR/IS peak area ratio of a standard sample.
  2. Plant extract (100 mg FW) and acetonitrile were both spiked with five BRs at 0.1 ng/mL.