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Table 4 Effect of GA20ox1 overexpression on the maize cell length profile

From: LEAF-E: a tool to analyze grass leaf growth using function fitting

Parameter AtGA20ox1 OE (mean ± SE) Control (mean ± SE) Difference in mean(+)
Cell length Lb (μm) 13.2 ± 1.7 10.9 ± 0.3 22% NS
Lm (μm) 118.1 ± 0.6 118.5 ± 3.8 0% NS
Position along the leaf axis Pm (mm) 29.5 ± 2.1 21.3 ± 0.8 38% **
Pe (mm) 56.6 ± 2.5 43.4 ± 0.3 -30% **
Zones along the leaf axis division zone (cell < =40 μm) (mm) 19.5 ± 0.5 14.6 ± 0.3 33% **
elongation zone (cell > 40 μm - Pe) (mm) 37.1 ± 1.0 28.7 ± 0.9 -29% **
  1. A segregating population produced by backcrossing (BC) a transgenic plant overexpressing the Arabidopsis thaliana GIBBERELLIC ACID 20 OXIDASE1 (GA20ox1)gene to the wild-type line B104 was analyzed for the cell lengths profile along the leaf axis. The results are based on the analysis of three transgenic and three non-transgenic BC1 plants. Lb: initial cell length Lm: final cell length; Pm: position along the leaf axis with maximal cell elongation rate; Pe: position along the leaf axis where cells reach their final length.
  2. +Statistical significance based on student t-test of non-transgenic plants (n = 3) vs GA20ox1 overexpression (n = 3), **p < 0.01, NS non-significant.
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Plant Methods

ISSN: 1746-4811