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Figure 2 | Plant Methods

Figure 2

From: A novel method to follow meiotic progression in Arabidopsis using confocal microscopy and 5-ethynyl-2′-deoxyuridine labeling

Figure 2

Confocal images of EdU labeled meiocytes. Detection of 5- ethynyl-2′-deoxyuridine(EdU) labeled. Arabidopsis pollen mother cells with Alexa Fluor 488 azide (green). The following prophase I substages are included: A) leptotene, B) zygotene, C) pachytene, D) diplotene, E) diakinesis. DNA is counterstained with propidium iodide (PI) (red). Each image is a single xy slice from a confocal z-stack. The PI stained nucleolus is not seen in these single slices but is always evident and used for staging when the entire z stack is analyzed . It should also be noted that only 3 of 5 bivalents can be seen in the diakinesis optical slice shown. Scale bar 5 um.

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