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Figure 9 | Plant Methods

Figure 9

From: Bacterially produced Pt-GFP as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic pH in intact Avena sativa and Vicia faba

Figure 9

Nitrate nutrition alkalized the leaf apoplast into Pt -GFP’s range of responsiveness. Comparison between the responsiveness of Pt-GFP (grey) and Oregon Green (black) to apoplastic pH changes as induced by the addition of 25 mM Cl− via L-cysteinium chloride to the roots of Vicia faba. Time point of chloride addition is indicated by the arrow. Plants were cultivated with 15 mM nitrate in the nutrient solution given as Ca(NO3)2. pH as quantified at the adaxial face of Vicia faba leaves is plotted over time. Leaf apoplastic pH was averaged (n = 6 ROIs per ratio image and time point; mean ± SE of ROIs). Representative kinetics of six equivalent recordings of plants gained from independent experiments (n = 6 biological replicates).

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Plant Methods

ISSN: 1746-4811