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Figure 8 | Plant Methods

Figure 8

From: Bacterially produced Pt-GFP as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic pH in intact Avena sativa and Vicia faba

Figure 8

In vivo calibration of Pt -GFP fluorescence ratio (F 490 /F 387 ). The Boltzmann fit was chosen for fitting sigmoidal curves to calibration ratio data. Fitting resulted in an optimal dynamic range for pH measurements between 5.3 and 8.4. In vivo calibration was conducted on six different plants, each biological replicate was technically replicated. Data are mean of n = 6 ± SE.

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Plant Methods

ISSN: 1746-4811