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Figure 2 | Plant Methods

Figure 2

From: Bacterially produced Pt-GFP as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic pH in intact Avena sativa and Vicia faba

Figure 2

Apoplastic distribution of the Pt -GFP in a Vicia faba leaf infiltrated 2.5 h prior image acquisition. Pt GFP is exclusively located in the apoplast. Confocal image in (A) shows adaxial view on palisade cell chloroplasts (exited at 633 nm; pseudo-red). Image in (B) shows same detail with Pt-GFP (excited at 488 nm; pseudo-yellow). (C) Overlay of (A) and (B) verifies the apoplastic distribution of the Pt-GFP that is attached outside of the palisade cells and, by this means, outlines the cell boundaries at 2.5 hours after loading. No Pt-GFP signal is emitted from between the chloroplasts, proofing that no Pt-GFP had entered the cytosol. Symplastic Pt GFP location was negated in several leaves derived from different plants. ‡, palisade cells.

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Plant Methods

ISSN: 1746-4811