Using monoclonal antibodies to label living root hairs: a novel tool for studying cell wall microarchitecture and dynamics in Arabidopsis

Table 1 Summary of antibodies employed and results of immunocytochemical labeling

mAb	Specificity/Reference	WT	vti13	prp3	prp3/vti13	csld2	csld3-2	xxt1	xxt2	xxt5
*PECTINS*
JIM7	High DE HG[31]	-	-	-	-	-	NA	-	-	-
LM19	Low DE HG[23]	++	++	+	++	-	NA	+	+	+
LM5	(1–4)-galactan[32]	-	-	-	-	-	NA	-	-	-
LM6	(1–5)-arabinan[33]	-	-	-	-	-	NA	-	-	-
INRA-RU1	Rhamnogalacturonan-I[34]	-	-	-	-	-	NA	-	-	-
*XYLOGLUCAN*
LM15	xylosylated XyG[29]	++	-	++	++	+	NA	-	-	-
LM25	galactosylated XyG epitope[20]	++	-	+	++	+	NA	-	-	-
*MANNAN*
BS-400-4	ß(1–4)-mannan/galacto-ß(1–4)-mannan[35]	-	-	-	-	-	NA	-	-	-
*XYLAN*
LM10	(1–4)-xylan[36]	++	-	++	++	++	NA	-	++	++
LM11	ß(1–4)-xylan/arabinoxylan[36]	+	-	+	+	-	NA	-	-	-
*AGP*
LM2	AGP[37]	-	-	-	-	-	NA	-	-	-
JIM13	AGP[38]	+	+	+	+	+	NA	+	+	+
JIM8	AGP[39]	+	+	+	+	+	NA	+	+	-
*EXTENSIN*
LM1	Extensin[40]	++	++	+	++	+	NA	+	+	+
JIM20	Extensin[41]	+	+	+	+	+	NA	+	+	+

Key: ++ = intense labeling, + = moderate labeling, - = ss no label, NA = not applicable, no root hairs formed.

ISSN: 1746-4811