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Table 1 Summary of antibodies employed and results of immunocytochemical labeling

From: Using monoclonal antibodies to label living root hairs: a novel tool for studying cell wall microarchitecture and dynamics in Arabidopsis

mAb Specificity/Reference WT vti13 prp3 prp3/vti13 csld2 csld3-2 xxt1 xxt2 xxt5
PECTINS           
JIM7 High DE HG[31] - - - - - NA - - -
LM19 Low DE HG[23] ++ ++ + ++ - NA + + +
LM5 (1–4)-galactan[32] - - - - - NA - - -
LM6 (1–5)-arabinan[33] - - - - - NA - - -
INRA-RU1 Rhamnogalacturonan-I[34] - - - - - NA - - -
XYLOGLUCAN           
LM15 xylosylated XyG[29] ++ - ++ ++ + NA - - -
LM25 galactosylated XyG epitope[20] ++ - + ++ + NA - - -
MANNAN           
BS-400-4 ß(1–4)-mannan/galacto-ß(1–4)-mannan[35] - - - - - NA - - -
XYLAN           
LM10 (1–4)-xylan[36] ++ - ++ ++ ++ NA - ++ ++
LM11 ß(1–4)-xylan/arabinoxylan[36] + - + + - NA - - -
AGP           
LM2 AGP[37] - - - - - NA - - -
JIM13 AGP[38] + + + + + NA + + +
JIM8 AGP[39] + + + + + NA + + -
EXTENSIN           
LM1 Extensin[40] ++ ++ + ++ + NA + + +
JIM20 Extensin[41] + + + + + NA + + +
  1. Key: ++ = intense labeling, + = moderate labeling, - = ss no label, NA = not applicable, no root hairs formed.