Figure 2

Live cell LM15 labeling of wild type (wt) Arabidopsis root hairs. A: Overview of live root demonstrating strong LM15 labeling of root hairs (arrows) and epidermis (hollow arrow). Bar = 100 μm. B: Magnified view of a root hair labeled with LM15 (arrow). Bar = 19 μm. C and D: LM15 labeling of root hair highlighting a slight but notable gradient with more intense labeling at the root hair tip (C, arrow). D is the DIC profile of C. Bars for C and D = 12 μm. E and F: LM15 labeling of root hair (E, arrow) after incubation of root in 0.085 mM Triton-X detergent (+D), during labeling process. Note labeling of root hair is comparable to that observed with non-detergent labeling (A-C). F is the DIC profile of E. Bars for E and F = 30 μm. G and H: LM15 labeling after root was pre-incubated overnight in 5U of xyloglucanase (+Xygase). Note the lack of labeling of root and hairs (G). H is the DIC profile of G. Bars for G and H = 18 μm. I and J: LM15 labeling after root was pre-incubated overnight in 3U of pectate lyase (+PLase). Note that the root hair labels (I, solid arrow). The epidermal cells labeling appears in strips (I, hollow arrow) but still labels. J is the DIC profile of I. Bars for I and J = 50 μm. K: Immunogold profile of root hair cell wall labeled with LM15 (arrow), Bar = 50 nm. L and M: Control profile whereby the primary antibody was eliminated during labeling. M is the DIC profile of L. Bars for L and M = 30 μm.