Figure 1

Congo Red fluorescence correlates with pollen tube extension. A) Schematic of workflow for measuring pollen tube extension using Congo Red. B) Fluorescence (arbitrary units; excitation: 470 nm, emission: 600 nm) is plotted against incubation time for pollen incubated in 2% DMSO (normal pollen tube extension) or 10% DMSO (no pollen tube extension, grains only). Error bars represent the standard deviation of at least three replicate experiments. C) Micrographs (DIC, left; Fluorescence, right) of pollen incubated in 2% or 10% DMSO for the indicated times (h, hours) and stained with Congo Red.