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Table 4 PCR reaction solution

From: Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue

Component Stock conc. Final conc./amount 1× (μL, in-tube) 1× (μL, on-slide)
HiFi Phusion buffer 10 20
dNTPs 10 mM 0.2 mM 1 2
DIG-11-dUTP 1 mM 4 μM 0.2 0.4
HiFi Phusion polymerase 2 U μL−1 1 U 0.5 0.5
Sterile water    33.3 67.1