Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue

Table 4 PCR reaction solution

Component	Stock conc.	Final conc./amount	1× (μL, in-tube)	1× (μL, on-slide)
HiFi Phusion buffer	5×	1×	10	20
dNTPs	10 mM	0.2 mM	1	2
DIG-11-dUTP	1 mM	4 μM	0.2	0.4
HiFi Phusion polymerase	2 U μL⁻¹	1 U	0.5	0.5
Sterile water			33.3	67.1

ISSN: 1746-4811