Table 2 Various alkaline phosphatase (AP) substrates used with this in situ PCR protocol on different plant species and tissues
From: Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
Plant species | Tissue | AP substrate |
|---|---|---|
Arabidopsis thaliana | Leaf | BM Purple |
Elf97 | ||
Vector Blue | ||
Arabidopsis thaliana | Flower | BM Purple |
Cleome gynandra | Leaf | BM purple |
Cleome hassleriana | Leaf | BM purple |
Glycine max | Root | BM purple |
Hordeum vulgare | Leaf | BM purple |
Elf97 | ||
Hordeum vulgare | Epidermal peel | BM Purple |
Elf97++ | ||
Oryza sativa | Leaf | BM Purple |
Vector Blue | ||
Oryza sativa | Root | BM Purple |
Solanum lycopersicum | Leaf | Vector Blue |
Triticum aestivum | Root | BM Purple |
Triticum durum | Leaf | Elf97 |
Triticum durum | Root | BM Purple |
NBT/BCIPX | ||
Vector Blue++ | ||
Vitis vinifera | Root | Vector Blue |
Vitis vinifera | Leaf | BM Purple |
Zea mays | Leaf | BM Purple |