Figure 3
From: Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
In situ PCR demonstrating vasculature-specific expression of a sodium transporter ( TmHKT1;5-A) in durum wheat ( Triticum durum ) root sections. The blue stain indicates the presence of transcripts while the images in the right panel are magnifications of figures in the left panel. (A) shows that TmHKT1;5-A is undetectable in Tamaroi [−Nax2] (the near-isogenic line without Nax2/TmHKT1;5-A). (B) depicts the stele-specific expression of TmHKT1;5-A in Tamaroi [+Nax2]. In (C) the expression of 18S ribosomal RNA is seen in all cell-types (positive control). (D) is the negative control where the reverse transcription (RT) step was omitted in Tamaroi [+Nax2]. c, cortex; en, endodermis; p, pericycle; x, xylem; xp, xylem parenchyma. Scale bars represent 100 μm. This figure was originally published in [21].