Confirmation of the insertion allele of At5g41740 in the GABI-Kat line 683F05. In line 683F05 two paralogous loci were predicted as possible insertion sites. Initiated by a user request for At5g41750, both loci were examined with primers specific for the respective paralogous locus. As a result, the worse prediction could be confirmed successfully (A) and the PCR for the better prediction failed (B). FST-based insertion site predictions are shown with blue (confirmed) and red (failed) triangles. Primer positions are given using dark red arrowheads. The affected genes are symbolised by blue arrows. Light blue arrows are 5′-UTRs, CDSs are shown in dark blue. Introns are indicated by thin blue lines. BLAST hits of the FSTs are shown as green arrows, BLAST hits of the confirmation sequences are shown as orange arrows. Sequence parts of FSTs and confirmation sequences that do not fit to the indicated positions are indicated as thin black lines. The genomic positions in the figure are given according to the TAIRv10 genome sequence dataset of A. thaliana.