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Table 1 Closest BLAST matches against NCBI- database of Sequences recovered from isolated Arctic Ocean samples

From: A novel cost effective and high-throughput isolation and identification method for marine microalgae

SampleID % < QV20 Closest species BLAST search hits Last certain common taxonomic assignment Times isolated NCBI Sequence ID Score Expect Identities Gaps
1-80-1-M 0 Uncultured marine eukaryote Class: Bacillariophyceae   GU385607.1 599 (324) 3.00E-167 324/324(100%) 0/324(0%)
   Bacillariophyta sp.    KF177731.1 593 (321) 2.00E-165 323/324(99%) 0/324(0%)
   Nitzschia thermalis    AY485458.1 588 (318) 7.00E-164 322/324(99%) 0/324(0%)
   Amphora sp.    AY485451.1 588 (318) 7.00E-164 322/324(99%) 0/324(0%)
  0 Cylindrotheca closterium Species: closterium   HM070405.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
1-80-3-S 0- 0.30 Cylindrotheca closterium Species: closterium 3 HM070405.1 608 (329) 6.00E-170 329/329(100%) 0/329(0%)
1-80-5-M 0.93 Cylindrotheca closterium Species: closterium   HM070405.1 597 (323) 1.00E-166 323/323(100%) 0/323(0%)
2-80-8-M 0 Skeletonema marinoi (5) Species: marinoi   HM805045.1 665 (360) 0 360/360(100%) 0/360(0%)
1-80-15-M 0.61 Fragilariaceae sp. Family: Fragilariaceae   JF794051.1 608 (329) 6.00E-170 329/329(100%) 0/329(0%)
   Synedra hyperborea Grunow    HQ912621.1 608 (329) 6.00E-170 329/329(100%) 0/329(0%)
   Synedra minuscula    EF423415.1 608 (329) 6.00E-170 329/329(100%) 0/329(0%)
   Fragilaria sp.    EU090021.1 608 (329) 6.00E-170 329/329(100%) 0/329(0%)
   Fragilaria cf. striatula    AJ971377.1 608 (329) 6.00E-170 329/329(100%) 0/329(0%)
  0 Cylindrotheca closterium Species: closterium   HM070405.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
2-80-27-M 0.30 Uncultured marine eukaryote Class: Bacillariophyceae   GU385607.1 610 (330) 2.00E-170 330/330(100%) 0/330(0%)
   Bacillariophyta sp.    KF177731.1 604 (327) 7.00E-169 329/330(99%) 0/330(0%)
   Nitzschia thermalis    AY485458.1 599 (324) 3.00E-167 328/330(99%) 0/330(0%)
   Amphora sp.    AY485451.1 599 (324) 3.00E-167 328/330(99%) 0/330(0%)
1-80-30-M 0- 0.31 Fragilariaceae sp. Family: Fragilariaceae 3 JF794051.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
   Synedra hyperborea    HQ912621.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
   Synedra minuscula    EF423415.1 595(322) 4.00E-166 322/322(100%) 0/322(0%)
   Fragilaria sp.    EU090021.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
   Fragilaria cf. striatula    AJ971377.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
1-80-37-M 0.31 Synedropsis cf. recta Family: Fragilariaceae   HQ912616.1 584 (316) 1.00E-162 318/319(99%) 0/319(0%)
   Fragilaria striatula    EU090018.1 584 (316) 1.00E-162 318/319(99%) 0/319(0%)
   Fragilaria barbararum    AJ971376.1 584 (316) 1.00E-162 318/319(99%) 0/319(0%)
2-80-51-M 0- 0.62 Chaetoceros cf. neogracile cf. species: neogracile 2 JN934684.1 595 (322) 4.00E-166 322/322(100%) 0/322(0%)
  0.55 Cylindrotheca closterium Species: closterium   HM070405.1 667 (361) 0 361/361(100% 0/361(0%)
2-80-61-M 0.94 Uncultured Chlorophyta Class: Chlorophyceae   FN690710.1 582 (315) 3.00E-162 317/318(99%) 0/318(0%)
   Chlamydomonas raudensis    AJ781313.1 555 (300) 7.00E-154 312/318(98%) 0/318(0%)
NA 0 Cylindrotheca closterium Species: closterium   HM070405.1 606 (328) 2.00E-169 328/328(100%) 0/328(0%)
  0 Synedropsis cf. recta Family: Fragilariaceae   HQ912616.1 636 (344) 3.00E-178 346/347(99%) 0/347(0%)
   Fragilaria striatula    EU090018.1 636 (344) 3.00E-178 346/347(99%) 0/347(0%)
   Fragilaria barbararum    AJ971376.1 636 (344) 3.00E-178 346/347(99%) 0/347(0%)
  0 Cylindrotheca closterium Species: closterium   HM070405.1 665 (360) 0 360/360(100%) 0/360(0%)
  0.62 Skeletonema marinoi Species: marinoi   HM805045.1 597 (323) 1.00E-166 323/323(100%) 0/323(0%)
  0 Uncultured Chlorophyta Class: Chlorophyceae 2 FN690710.1 588 (318) 7.00E-164 320/321(99%) 0/321(0%)
   Chlamydomonas raudensis    AJ781313.1 560 (303) 2.00E-155 315/321(98%) 0/321(0%)