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Figure 1 | Plant Methods

Figure 1

From: Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies

Figure 1

Laser scanner imaging of fluorescent protein-expressing leaves. Fluorescent proteins were transiently expressed by co-infiltrating N. benthamiana leaf tissue with an Agrobacterium pSN.5 p19 culture plus cultures of Agrobacterium containing pBin-35S-mGFP5 (mGFP5), pSN.5 TagRFP-T (TagRFP-T), pSN.5 mTFP1 (mTFP1) or pSN.5 mPapaya1 (mPapaya1). Fluorescence was imaged by leaf laser scanning. (A) Signal acquired at 6 dpa for TagRFP-T (red) and mGFP5 (green); green and red channel images were merged. Scale bar, 2 cm. (B) Signal at 3 dpa for mTFP1 (cyan) and mPapaya1 (yellow); cyan and yellow channel images were merged. Scale bar, 2 cm. (C,D) Surface plots of infiltrated patches from above images.

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Plant Methods

ISSN: 1746-4811