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Figure 1 | Plant Methods

Figure 1

From: Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species

Figure 1

Genomic DNA preparation of Corymbia citriodora subsp. variegata resolved by electrophoresis. 1 kb DNA ladder (1, 14), 100 and 200 ng λ DNA standards (2, 3 and 12, 13) respectively. DNA extractions using the traditional CTAB-based method with no PVP (4), 1% PVP (5), and 4% PVP (6). DNA extractions using the NGS protocol with no PVP (7), 1% PVP (8), and 4% PVP (9). Endonuclease digestions of DNA extracted without PVP with Eco RI (10) and High-Fidelity Hin dIII (11). Results from six grams of leaf tissue finely ground using a mortar and pestle, then aliquoted (1 g) for each extraction. DNA was resolved by electrophoresis in a 0.7% agarose gel and visualized using SYBR Safe DNA gel stain. Percentages are represented as w/v. CTAB: hexadecyltrimethylammonium bromide; PVP: polyvinylpyrrolidone.

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Plant Methods

ISSN: 1746-4811