Figure 8

Monitoring Arabidopsis circadian rhythm by transient expression of GIGANTEA ::luciferase ( GI :: LUC2 ). Four-day-old Arabidopsis efr-1 seedlings were infected with Agrobacterium C58C1(pTiB6S3ΔT)^H carrying a vector (pCAMBIA1390) or p1390-GI-LUC2 by the AGROBEST method for 3 days in a 16-h/8-h light/dark cycle (75 μmol m^-2 s^-1), then transferred to 1/2 MS liquid medium in the presence of 100 μM Timentin and 0.5 mM luciferin and grown under continuous light at 40 μmol m^-2 s^-1 for up to 5 days. The GI::LUC2 transgenic Arabidopsis plant (TP) cultured in identical conditions without Agrobacterium infection was a positive control. Real-time bioluminescence signals were photographed and the luciferase intensity is shown as mean ± SEM from 12 seedlings expressing GI::LUC2. Similar results were obtained from at least 3 independent experiments. The white and gray regions indicate subjective light and dark periods, respectively.