Transient transformation events in different organs and cell types. (A-D) Four-day-old (A and C-D) or 7-d-old (B) Arabidopsis efr-1 seedlings were infected with C58C1(pTiB6S3ΔT)H carrying pBISN1 by the AGROBEST method and analyzed for GUS staining. GUS staining was detected in true leaves (B, indicated by asterisk), cotyledons (A and B), main roots near lateral initiation site (C), and elongation zone (D). (E-L) Confocal microscopy of 4-day-old Arabidopsis efr-1 seedlings infected with C58C1(pTiB6S3ΔT)H carrying various vectors for transient expression of indicated fluorescent proteins by the ABM200 method. Fluorescence signals for 35S::Venus-intron or 35S::NLS-RFP were detected in cotyledons (E and F). Venus-intron signals were detected in different types of cells, including epidermal cells (G), guard cells (H), mesophyll cells (I) of cotyledon, and root epidermal cells (J). (K) Subcellular localization of Venus-intron and NLS-RFP by co-infection of 2 Agrobacterium strains expressing 35S::Venus-intron or 35S::NLS-RFP. (L) Protein–protein interaction by BiFC of nYFP-ASK1 and TIR1-cYFP. Images show fluorescence alone (K) and/or merged with bright field (E, F, J and L) or chloroplast fluorescence (G-I). Scale bars are 2 mm (A and B), 0.5 mm (C and D), 100 μm (E, F and J), 50 μm (L) and 20 μm (G-I and K). BiFC, bimolecular fluorescence complementation.