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Figure 10 | Plant Methods

Figure 10

From: AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings

Figure 10

Transient expression of MYB75 increases anthocyanin accumulation in Col-0 seedlings. Four-day-old Arabidopsis Col-0 seedlings were infected with Agrobacterium C58C1(pTiB6S3ΔT)H carrying a vector (pCAMBIA1390), 2X35S::MYB75, or super::gusA-intron by the AGROBEST method. At 3 dpi, co-cultivation medium was replaced with MS medium containing 100 μM timemtin for additional incubation for 3 days. qRT-PCR of relative expression of MYB75 (A) and CHS (B) with representative data shown with mean ± SD from 3 technical repeats. Similar results were obtained from three independent experiments. Zeiss inverted microscopy of anthocyanin accumulation in seedlings (upper panels) and cotyledons (lower panels) and quantification (C). Data for anthocyanin content are mean ± SD from 3 independent experiments (20–30 seedlings for each biological repeat, 3 biological repeats for each independent experiment). Values significantly different from those obtained with vector are denoted (**P < 0.01, by Student’s t test).

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Plant Methods

ISSN: 1746-4811