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Table 1 Summary of V MAX, K CAT and K M values of the HIC purified Rubisco fraction from S. oleracea and B. oleracea extracts

From: Isolation and kinetic characterisation of hydrophobically distinct populations of form I Rubisco

Species [(NH4)2SO4] mM VMAX (μmol.min-1.mgRubisco-1) KCAT*(s-1) KM (RuBP) (μM)
S. oleracea 400 2.31 ± 0.16 2.63 ± 0.20 44 ± 11
  300 2.20 ± 0.11 2.52 ± 0.14 39 ± 7
  200 2.35 ± 0.07 2.76 ± 0.10 39 ± 4
  0 2.23 ± 0.06 2.59 ± 0.08 45 ± 6
B. oleracea 400 0.94 ± 0.08 1.09 ± 0.09 147 ± 35
  300 1.02 ± 0.11 1.17 ± 0.13 92 ± 29
  200 1.16 ± 0.09 1.34 ± 0.10 83 ± 19
  0 1.21 ± 0.10 1.39 ± 0.11 77 ± 19
  1. *KCAT was calculated based on 8 active sites and a molecular weight of 550 K g/mol.