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Table 1 Summary of V MAX, K CAT and K M values of the HIC purified Rubisco fraction from S. oleracea and B. oleracea extracts

From: Isolation and kinetic characterisation of hydrophobically distinct populations of form I Rubisco

Species

[(NH4)2SO4] mM

VMAX (ÎĽmol.min-1.mgRubisco-1)

KCAT*(s-1)

KM (RuBP) (ÎĽM)

S. oleracea

400

2.31 ± 0.16

2.63 ± 0.20

44 ± 11

 

300

2.20 ± 0.11

2.52 ± 0.14

39 ± 7

 

200

2.35 ± 0.07

2.76 ± 0.10

39 ± 4

 

0

2.23 ± 0.06

2.59 ± 0.08

45 ± 6

B. oleracea

400

0.94 ± 0.08

1.09 ± 0.09

147 ± 35

 

300

1.02 ± 0.11

1.17 ± 0.13

92 ± 29

 

200

1.16 ± 0.09

1.34 ± 0.10

83 ± 19

 

0

1.21 ± 0.10

1.39 ± 0.11

77 ± 19

  1. *KCAT was calculated based on 8 active sites and a molecular weight of 550 K g/mol.
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Plant Methods

ISSN: 1746-4811