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Figure 6 | Plant Methods

Figure 6

From: Isolation and kinetic characterisation of hydrophobically distinct populations of form I Rubisco

Figure 6

Kinetic and structural characterisation of four HIC purified Rubisco fractions isolated from S. oleracea extracts. (A) Determination of the KM and VMAX values for the substrate RuBP for B. oleracea HIC purified fractions; Average VMAX values obtained for the 400, 300, 200 and 0 mM HIC fractions were 0.94 ± 0.08, 1.02 ± 0.11, 1.16 ± 0.09 and 1.21 ± 0.10 μmol.min-1.mgRubisco-1 respectively. Average KM values obtained were 147 ± 35, 92 ± 29, 83 ± 19 and 77 ± 19 μM respectively. Kinetic calculations and curve-fitting was done using GraphPad Prism 6 software. Error bars shown are standard deviation with n = 6 (3 different biological replicates measured in duplicate). (B) Circular dichroism spectra of B. oleracea. Rubisco fractions purified using HIC; The 400 mM, 300 mM, 200 mM and 0 M Rubisco fractions were was run at 25°C and then at 80°C, at concentration between 0.15-0.25 mg/mL. Data are shown as an average molar ellipticity [θ] of 3 biological repeats.

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