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Figure 1 | Plant Methods

Figure 1

From: Isolation and kinetic characterisation of hydrophobically distinct populations of form I Rubisco

Figure 1

SDS-PAGE gels and western blots showing protein content in ammonium sulphate fractions from S. oleracea and B. oleracea. (A) SDS-PAGE gel showing protein content in ammonium sulphate fractions from S. oleracea; Extracts were subjected to ammonium sulphate precipitation as described in Methods. Samples were separated by SDS-PAGE and subsequently stained for protein. The position of Rubisco’s large (LS) and small (SS) subunits are indicated in the figure. 0.4 μg of sample was added per lane. (B) Western blot showing Rubisco content in ammonium sulphate fractions from S. oleracea; The relative intensities of the LS were calculated to be 0.85 ± 0.04 (CE), 0.89 ± 0.01(8000 × g), 0.92 ± 0.02 (35% SN), 0.55 ± 0.15 (35% pell) and 1 (60% pell). (C) SDS-PAGE gel showing protein content in ammonium sulphate fractions from B. oleracea; Extracts were subjected to ammonium sulphate precipitation as described in Methods. Samples were separated by SDS-PAGE and subsequently stained for protein. The position of Rubisco’s large (LS) and small (SS) subunits are indicated in the figure. 0.6 μg of sample was added per lane. (D) Western blot showing Rubisco content in ammonium sulphate fractions from B. oleracea; The relative intensities of the LS were calculated to be 0.83 ± 0.06 (CE), 0.85 ± 0.03 (8000 × g), 0.89 ± 0.06 (35% SN), 0.61 ± 0.12 (35% pell) and 1 (60% pell).

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