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Figure 3 | Plant Methods

Figure 3

From: A virus-induced gene silencing (VIGS) system for functional genomics in the parasitic plant Striga hermonthica

Figure 3

RT-PCR to confirm silencing of PDS gene on S. hermonthica. The PDS and TRV gene specific primers were used. (a) S. hermonthica treated with GV3101 with the TRV2 vector containing the PDS gene insert (GV3101/TRV2-PDS) + GV3101/TRV1) and amplified with PDS primers. Lane (M) represents 1 kb ladder while 1, 2, and 3 are the replicates. (b) The cDNA plants treated with GV3101 containing TRV1 empty vectors (GV3101/TRV1) with three replicates (1, 2 and 3) and amplified with PDS primers. (c) Three replicates (1, 2, 3) of plants treated with GV3101 empty and amplified with PDS primers. (d) Represents plants treated only with water and amplified with PDS primers. (e) S. hermonthica cDNA amplified with TRV primers from plants treated with GV3101/TRV2-PDS + GV3101/TRV1 vectors with three replicates (1, 2, 3). (f) S. hermonthica cDNA from Plants treated with GV3101/TRV1 empty vectors, three replicates (1, 2, and 3) amplified with TRV primers. (g) Plants treated with GV3101 empty, three replicates (1, 2, 3) amplified with TRV primers. (h) Plants treated with water only, three replicates (1,2,3), cDNA amplified with TRV primers. Images (i, j, k and l) are the internal control using Actin primers for the S. hermonthica cDNA from plants treated with GV3101/TRV2-PDS + GV3101/TRV1, Plants treated with GV3101/TRV1 only, Plants treated with GV3101 only and plants treated with water only, respectively.

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