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Figure 2 | Plant Methods

Figure 2

From: Fluorescent labelling of the actin cytoskeleton in plants using a cameloid antibody

Figure 2

Golgi movement and actin cytoskeleton dynamics. A) Transient co-expression of the actin-chromobody (YFP-actin-Cb in yellow) construct with the Golgi marker ST-GFP (green dots); B) co-expression of Lifeact GFP (green lines) with ST-GFP (green dots). Scale bars = 5 μm. Cumulative Distribution Frequency (CDF) plots of velocity (C) and displacement rate (D) of N. tabacum transiently expressing only ST-GFP (blue line), both ST-GFP and YFP-actin-Cb (yellow line) or ST-GFP and Lifeact-GFP (green line), respectively. Curves marked with shapes (*, ♦) indicate a statistically significant difference from the control ST-GFP of p < 0.05. E) Velocity, displacement rate and meandering index values calculated with Volocity software for N. tabacum transiently expressing ST-GFP (blue line), both ST-GFP and YFP-actin-Cb (yellow line) or ST-GFP and Lifeact-GFP (green line), respectively. Mean values are expressed as a percentage of the control (ST-GFP). Symbols (*, ♦, ▲) indicate that the means are significantly different from the control at p < 0.05. F) Schematic representation of the path and movement pattern of Golgi bodies. When ST-GFP is coexpressed with Lifeact-GFP, Golgi bodies move same linear distance as the control but have a less salutatory path. The coexpression of the antibody determines Golgi bodies moving shorter linear distance and slightly more salutatory.

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