Transient expression of the actin-chromobody. Lane 1) Transient expression of the actin-chromobody (Actin-Cb) construct with a C-terminal YFP-fusion in Nicotiana tabacum leaves (A); co-expression with the endoplasmic reticulum marker HDEL fused to GFP is shown (B, C). Lane 2) Transient expression of the actin-chromobody (actin-Cb) construct with an N-terminal YFP-fusion in Nicotiana tabacum leaves at different Agrobacterium concentrations: OD600 = 0.1 (A), OD600 = 0.0.5 (B), OD600 = 0.01 (C). Lane 3) Depolymerisation of actin cytoskeleton (labelled by the actin-chromobody) after 15 in (A), 30 min (B) and (45 min (C) treatment with 25 μM latrunculin B (LatB). Lane 4) Repolymerisation of the actin cytoskeleton by washing out the LatB after 30 min (A), 45 min (B) and 60 min (C), respectively.