Keel petal incision: a simple and efficient method for genetic crossing in Medicago truncatula

Table 2 Confirmed successful crosses

Crossing parents, mutant as female	Total number of plants tested	Number of confirmed plants by nodule phenotyping	Success rate,%
NF11217 X R108	28	28	100
NF11044 X R108	31	31	100
NF8324 X R108	17	17	100
NF1320 X R108	14	11	78
Crossing parents, wild-type as female	Total number of plants tested	Number of confirmed plants by PCR genotyping	Success rate,%
R108 X NF10796	8	8	100
R108 X NF8324	13	11	84
A17 X NF8324	8	8	100
A20 X NF8324	11	8	72

For each cross, the first parent listed in the cross was used as the female. For cases where the mutant served as female in the cross, successful crossing was confirmed by the appearance of pink nodules in the BC₁F₁ progeny. When wild-type served as female, including inter-ecotype crosses, crosses were confirmed in the BC₁F₁, or F₁ progeny by PCR genotyping using Tnt1 specific primers, as in Figure 7.

ISSN: 1746-4811