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Table 1 Estimation of crossing success via seed pod formation

From: Keel petal incision: a simple and efficient method for genetic crossing in Medicago truncatula

Crossing parents, wild-type as female

Attempted crosses

Seed pods formed

Success rate,%

R108 X A17

9

6

67

R108 X A20

14

13

93

R108 X NF10796

17

12

71

R108 X NF11014

22

19

86

R108 X NF11044

27

22

81

R108 X NF11166

7

7

100

R108 X NF11217

11

8

73

R108 X NF1320

38

29

76

R108 X NF1320-29-3

17

17

100

R108 X NF1320-BC1-F3

21

16

76

R108 X NF4619

5

4

80

R108 X NF8324

31

23

74

A17 X R108

43

41

95

A17 X NF11044

91

82

90

A17 X NF11217

30

26

87

A17 X NF8324

18

12

67

A20 X R108

70

63

90

A20 X NF11044

58

49

84

A20 X NF11217

11

10

91

A20 X NF1320

15

9

60

A20 X NF8324

29

21

72

Sub-total

584

489

84

Crossing parents, mutant as female

Attempted crosses

Seed pods formed

Success rate, %

NF10796 X R108

14

9

64

NF11014 X R108

16

11

69

NF11044 X R108

28

22

79

NF11166 X R108

4

4

100

NF11217 X R108

16

11

69

NF1320 X R108

30

23

77

NF8324 X A20

7

5

71

NF8324 X R108

34

27

79

Sub-total

149

112

75

Total crosses

733

601

82

  1. For each cross, the first parent listed in the cross served as the female. All crosses were performed by two graduate students, one with one year and the other with two years of research experience. Success rates were calculated based on whether a pod formed in the cross-pollinated flower buds ten days post-pollination.