Figure 7
From: Keel petal incision: a simple and efficient method for genetic crossing in Medicago truncatula
Confirmation of successful crosses by genotyping. A-C, Wild-type R108, NF10796 and NF8324, R108 x NF10796-BC1F1, A17 x NF8324-F1 and A20 x NF8324-F1 plants were PCR genotyped using Tnt1 transposon specific primers. D, Primers for the MtIRE-like gene were used in the PCR amplifications as positive control for the wild-type genomic DNA. Tnt1 DNA is not found in wild-type R108, A17 or A20 plants but can be detected in all F1 plants indicating successful crosses (A-C). Stained gel images have been cropped to show the PCR products of 612Â bp from Tnt1 and 422Â bp from MtIRE-like amplifications respectively.